bethyl recognizing phospho kap1 (Bethyl)
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Bethyl Recognizing Phospho Kap1, supplied by Bethyl, used in various techniques. Bioz Stars score: 94/100, based on 303 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 303 article reviews
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Incubation:Article Title: HIF-1-regulated TPM3 links hypoxia to motility and invasion beyond the hypoxic fraction in triple-negative breast cancer. Article Snippet: After a brief sonication, proteins were separated on a 4-20% polyacrylamide gel (Bio-Rad) and transferred onto a nitrocellulose membrane (Bio-Rad).After a brief sonication, proteins were separated on a 4-20% polyacrylamide gel (Bio-Rad) and transferred onto a nitrocellulose membrane (Bio-Rad).. Primary antibodies used were: TPM3 (Abcam, ab113692), - AR TI CL E IN P RE SS Zhou et al., actin (Santa Cruz, sc-69879), HIF-1 (BD Biosciences, 610958), GRP78 (BD Biosciences, 610979), KAP1 (Bethyl, A300-274A), Article Title: HIF-1–regulated TPM3 links hypoxia to motility and invasion beyond the hypoxic fraction in triple-negative breast cancer Article Snippet: After a brief sonication, proteins were separated on a 4-20% polyacrylamide gel (Bio-Rad) and transferred onto a nitrocellulose membrane (Bio-Rad).After a brief sonication, proteins were separated on a 4-20% polyacrylamide gel (Bio-Rad) and transferred onto a nitrocellulose membrane (Bio-Rad).. Primary antibodies used were: TPM3 (Abcam, ab113692), β-actin (Santa Cruz, sc-69879), HIF-1α (BD Biosciences, 610958), GRP78 (BD Biosciences, 610979), KAP1 (Bethyl, A300-274A), Article Title: Replication catastrophe induced by cyclic hypoxia leads to increased APOBEC3B activity Article Snippet: Experiments were carried out in triplicate and a representative blot is shown for each experiment.Experiments were carried out in triplicate and a representative blot is shown for each experiment.. Antibodies used; Cell Signaling: p53-S15 (9284), RPA32 (2208), Chk1-S345 (2341), Chk1-S296 (90178), H3 (3638), H3-S10 (9701), p65 (3033), p65-S536 (93H1), cGAS (15102); Santa Cruz: p53 (sc-126), β-Actin (sc-69879), Chk1 (sc-8408), ATR (sc-515173); Article Title: Replication Stress and Chromatin Context Link ATM Activation to a Role in DNA Replication Article Snippet: Cells were lysed in UTB (9 M urea, 75 mM Tris-HCl pH 7.5 and 0.15 M β-mercaptoethanol) and sonicated briefly.Cells were lysed in UTB (9 M urea, 75 mM Tris-HCl pH 7.5 and 0.15 M β-mercaptoethanol) and sonicated briefly.. Antibodies used were CHK2-T68, p53-S15, H3K9me2, Article Title: Targeting radiation-resistant hypoxic tumour cells through ATR inhibition Article Snippet: Cell monolayers or spheroids were lysed in UTB (9 ℳ urea, 75 mℳ Tris-HCl pH 7.5 and 0.15 ℳ β -mercaptoethanol) and sonicated briefly.Cell monolayers or spheroids were lysed in UTB (9 ℳ urea, 75 mℳ Tris-HCl pH 7.5 and 0.15 ℳ β -mercaptoethanol) and sonicated briefly.. Antibodies used were Chk2-T68, p53-S15, Chk1-S296, Chk1-S317 and Chk1-S345 (Cell Signaling Technologies, Danvers, MA, USA), |
