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bethyl recognizing phospho kap1  (Bethyl)


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    Structured Review

    Bethyl bethyl recognizing phospho kap1
    Bethyl Recognizing Phospho Kap1, supplied by Bethyl, used in various techniques. Bioz Stars score: 94/100, based on 303 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/kap1+s824/Phospho+KAP-1+(S824)+Antibody/pm41897352-64-71-71
    Average 94 stars, based on 303 article reviews
    bethyl recognizing phospho kap1 - by Bioz Stars, 2026-10
    94/100 stars

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    Incubation:

    Article Title: HIF-1-regulated TPM3 links hypoxia to motility and invasion beyond the hypoxic fraction in triple-negative breast cancer.
    Article Snippet: After a brief sonication, proteins were separated on a 4-20% polyacrylamide gel (Bio-Rad) and transferred onto a nitrocellulose membrane (Bio-Rad).After a brief sonication, proteins were separated on a 4-20% polyacrylamide gel (Bio-Rad) and transferred onto a nitrocellulose membrane (Bio-Rad).. Primary antibodies used were: TPM3 (Abcam, ab113692), - AR TI CL E IN P RE SS Zhou et al., actin (Santa Cruz, sc-69879), HIF-1 (BD Biosciences, 610958), GRP78 (BD Biosciences, 610979), KAP1 (Bethyl, A300-274A), KAP1-S824 (Bethyl, A300-767A), ALIX (Abcam, ab275377), PRDX3 (Abcam, ab73349), GM130 (Abcam, ab52649).. Secondary antibodies: IRDye 680RD goat anti-mouse IgG (LI-COR, 926-68070), IRDye 800CW Goat anti-rabbit IgG (LI-COR, 926- 32211), Goat anti-mouse IgG HRP (Invitrogen, 31430), Goat anti-rabbit IgG HRP (Invitrogen, 31460).Secondary antibodies: IRDye 680RD goat anti-mouse IgG (LI-COR, 926-68070), IRDye 800CW Goat anti-rabbit IgG (LI-COR, 926- 32211), Goat anti-mouse IgG HRP (Invitrogen, 31430), Goat anti-rabbit IgG HRP (Invitrogen, 31460).

    Article Title: HIF-1–regulated TPM3 links hypoxia to motility and invasion beyond the hypoxic fraction in triple-negative breast cancer
    Article Snippet: After a brief sonication, proteins were separated on a 4-20% polyacrylamide gel (Bio-Rad) and transferred onto a nitrocellulose membrane (Bio-Rad).After a brief sonication, proteins were separated on a 4-20% polyacrylamide gel (Bio-Rad) and transferred onto a nitrocellulose membrane (Bio-Rad).. Primary antibodies used were: TPM3 (Abcam, ab113692), β-actin (Santa Cruz, sc-69879), HIF-1α (BD Biosciences, 610958), GRP78 (BD Biosciences, 610979), KAP1 (Bethyl, A300-274A), KAP1-S824 (Bethyl, A300-767A), ALIX (Abcam, ab275377), PRDX3 (Abcam, ab73349), GM130 (Abcam, ab52649).. Secondary antibodies: IRDye 680RD goat anti-mouse IgG (LI-COR, 926-68070), IRDye 800CW Goat anti-rabbit IgG (LI-COR, 926-32211), Goat anti-mouse IgG HRP (Invitrogen, 31430), Goat anti-rabbit IgG HRP (Invitrogen, 31460).Secondary antibodies: IRDye 680RD goat anti-mouse IgG (LI-COR, 926-68070), IRDye 800CW Goat anti-rabbit IgG (LI-COR, 926-32211), Goat anti-mouse IgG HRP (Invitrogen, 31430), Goat anti-rabbit IgG HRP (Invitrogen, 31460).

    Article Title: Replication catastrophe induced by cyclic hypoxia leads to increased APOBEC3B activity
    Article Snippet: Experiments were carried out in triplicate and a representative blot is shown for each experiment.Experiments were carried out in triplicate and a representative blot is shown for each experiment.. Antibodies used; Cell Signaling: p53-S15 (9284), RPA32 (2208), Chk1-S345 (2341), Chk1-S296 (90178), H3 (3638), H3-S10 (9701), p65 (3033), p65-S536 (93H1), cGAS (15102); Santa Cruz: p53 (sc-126), β-Actin (sc-69879), Chk1 (sc-8408), ATR (sc-515173); Bethyl: KAP1 (A300-274A), KAP1-S824 (A300-767A); Millipore: γH2AX (05–636), H2AX (07–627); Abcam: A3B (ab184990), Cyclin A (ab181591); BD Biosciences: HIF-1α (610958), GRP78 (610979).

    Article Title: Replication Stress and Chromatin Context Link ATM Activation to a Role in DNA Replication
    Article Snippet: Cells were lysed in UTB (9 M urea, 75 mM Tris-HCl pH 7.5 and 0.15 M β-mercaptoethanol) and sonicated briefly.Cells were lysed in UTB (9 M urea, 75 mM Tris-HCl pH 7.5 and 0.15 M β-mercaptoethanol) and sonicated briefly.. Antibodies used were CHK2-T68, p53-S15, H3K9me2, KAP1-S824, (Bethyl), ATM-S1981 (Epitomics), ATM-S1981(Rockland), β-actin (Santa Cruz), H2AX and H3K9me3 (Upstate), HIF-1α BD Biosciences), β-tubulin, H3, H3K27me3 and HP1β (Abcam), pp44/42 T202-Y204 MAPK and p44/p42 MAPK (Cell Signaling).. The Odyssey infrared imaging technology was used (LI-COR Biosciences).The Odyssey infrared imaging technology was used (LI-COR Biosciences).

    Article Title: Targeting radiation-resistant hypoxic tumour cells through ATR inhibition
    Article Snippet: Cell monolayers or spheroids were lysed in UTB (9 ℳ urea, 75 mℳ Tris-HCl pH 7.5 and 0.15 ℳ β -mercaptoethanol) and sonicated briefly.Cell monolayers or spheroids were lysed in UTB (9 ℳ urea, 75 mℳ Tris-HCl pH 7.5 and 0.15 ℳ β -mercaptoethanol) and sonicated briefly.. Antibodies used were Chk2-T68, p53-S15, Chk1-S296, Chk1-S317 and Chk1-S345 (Cell Signaling Technologies, Danvers, MA, USA), KAP1-S824, (Bethyl Laboratories, Inc., Montgomery, AL, USA), ATM-S1981 (Epitomics, Inc., Burlingame, CA, USA), p53 and β -actin (Santa Cruz Biotechnology, Santa Cruz, CA, USA), γ H2AX (Upstate-Millipore, Billerica, MA, USA), HIF-1 α (BD Biosciences, Oxford, UK) and GLUT-1 (Abcam, Cambridge, UK).. The Odyssey infrared imaging technology was used (LI-COR Biosciences, Lincoln, NE, USA).The Odyssey infrared imaging technology was used (LI-COR Biosciences, Lincoln, NE, USA).



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    ( A ) HCT116 SIRT2 WT and KO cells were treated with 6 Gy IR, harvested after 4 hours, run on SDS-PAGE, and probed with the indicated antibodies. The image shown is representative of data from 3 separate experiments conducted independently. ( B ) Quantitation of IR-induced phosphorylation of <t>KAP1</t> and CHK2 relative to total KAP1 and CHK2 protein levels. Data are represented as the mean ± SD from 3 independent experiments. ** P < 0.01 and *** P < 0.001, by 2-tail Student’s t test. ( C ) HCT116 SIRT2 KO cells expressing SFB-MRE11 WT, K393Q, and K393R were treated with or without 6 Gy IR, harvested after 4 hours, run on SDS-PAGE, and probed with the indicated antibodies. Shown is a representative image of 3 independent experiments. ( D and E ) Quantitation of phosphorylation of KAP1 and CHK2 relative to total KAP1 and CHK2 protein levels. Data are presented as the mean ± SD of 3 independent experiments. Statistical significance was determined using 1-way ANOVA followed by Dunnett’s post hoc test for multiple comparisons. **** P < 0.0001.
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    ( A ) HCT116 SIRT2 WT and KO cells were treated with 6 Gy IR, harvested after 4 hours, run on SDS-PAGE, and probed with the indicated antibodies. The image shown is representative of data from 3 separate experiments conducted independently. ( B ) Quantitation of IR-induced phosphorylation of <t>KAP1</t> and CHK2 relative to total KAP1 and CHK2 protein levels. Data are represented as the mean ± SD from 3 independent experiments. ** P < 0.01 and *** P < 0.001, by 2-tail Student’s t test. ( C ) HCT116 SIRT2 KO cells expressing SFB-MRE11 WT, K393Q, and K393R were treated with or without 6 Gy IR, harvested after 4 hours, run on SDS-PAGE, and probed with the indicated antibodies. Shown is a representative image of 3 independent experiments. ( D and E ) Quantitation of phosphorylation of KAP1 and CHK2 relative to total KAP1 and CHK2 protein levels. Data are presented as the mean ± SD of 3 independent experiments. Statistical significance was determined using 1-way ANOVA followed by Dunnett’s post hoc test for multiple comparisons. **** P < 0.0001.
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    ( A ) HCT116 SIRT2 WT and KO cells were treated with 6 Gy IR, harvested after 4 hours, run on SDS-PAGE, and probed with the indicated antibodies. The image shown is representative of data from 3 separate experiments conducted independently. ( B ) Quantitation of IR-induced phosphorylation of <t>KAP1</t> and CHK2 relative to total KAP1 and CHK2 protein levels. Data are represented as the mean ± SD from 3 independent experiments. ** P < 0.01 and *** P < 0.001, by 2-tail Student’s t test. ( C ) HCT116 SIRT2 KO cells expressing SFB-MRE11 WT, K393Q, and K393R were treated with or without 6 Gy IR, harvested after 4 hours, run on SDS-PAGE, and probed with the indicated antibodies. Shown is a representative image of 3 independent experiments. ( D and E ) Quantitation of phosphorylation of KAP1 and CHK2 relative to total KAP1 and CHK2 protein levels. Data are presented as the mean ± SD of 3 independent experiments. Statistical significance was determined using 1-way ANOVA followed by Dunnett’s post hoc test for multiple comparisons. **** P < 0.0001.
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    Image Search Results


    ( A ) HCT116 SIRT2 WT and KO cells were treated with 6 Gy IR, harvested after 4 hours, run on SDS-PAGE, and probed with the indicated antibodies. The image shown is representative of data from 3 separate experiments conducted independently. ( B ) Quantitation of IR-induced phosphorylation of KAP1 and CHK2 relative to total KAP1 and CHK2 protein levels. Data are represented as the mean ± SD from 3 independent experiments. ** P < 0.01 and *** P < 0.001, by 2-tail Student’s t test. ( C ) HCT116 SIRT2 KO cells expressing SFB-MRE11 WT, K393Q, and K393R were treated with or without 6 Gy IR, harvested after 4 hours, run on SDS-PAGE, and probed with the indicated antibodies. Shown is a representative image of 3 independent experiments. ( D and E ) Quantitation of phosphorylation of KAP1 and CHK2 relative to total KAP1 and CHK2 protein levels. Data are presented as the mean ± SD of 3 independent experiments. Statistical significance was determined using 1-way ANOVA followed by Dunnett’s post hoc test for multiple comparisons. **** P < 0.0001.

    Journal: The Journal of Clinical Investigation

    Article Title: MRE11 deacetylation by SIRT2 promotes DNA binding to facilitate DNA end resection and ATM-dependent signaling

    doi: 10.1172/JCI186711

    Figure Lengend Snippet: ( A ) HCT116 SIRT2 WT and KO cells were treated with 6 Gy IR, harvested after 4 hours, run on SDS-PAGE, and probed with the indicated antibodies. The image shown is representative of data from 3 separate experiments conducted independently. ( B ) Quantitation of IR-induced phosphorylation of KAP1 and CHK2 relative to total KAP1 and CHK2 protein levels. Data are represented as the mean ± SD from 3 independent experiments. ** P < 0.01 and *** P < 0.001, by 2-tail Student’s t test. ( C ) HCT116 SIRT2 KO cells expressing SFB-MRE11 WT, K393Q, and K393R were treated with or without 6 Gy IR, harvested after 4 hours, run on SDS-PAGE, and probed with the indicated antibodies. Shown is a representative image of 3 independent experiments. ( D and E ) Quantitation of phosphorylation of KAP1 and CHK2 relative to total KAP1 and CHK2 protein levels. Data are presented as the mean ± SD of 3 independent experiments. Statistical significance was determined using 1-way ANOVA followed by Dunnett’s post hoc test for multiple comparisons. **** P < 0.0001.

    Article Snippet: The antibodies used in this study included SIRT2 (MilliporeSigma, 09-843; Santa Cruz Biotechnology, sc-20966), GAPDH (Santa Cruz, sc-47724), Flag (Santa Cruz Biotechnology, sc-51590), GFP (Abcam, Ab6556), pan acetyl-Lys (Santa Cruz Biotechnology, sc-8649), RPA70 (Cell Signaling Technology, 2267), P-CHK2 T68 (Cell Signaling Technology, 2661S), CHK2 (Santa Cruz Biotechnology, sc-7898), P-KAP1 (Invitrogen, Thermo Fisher Scientific, MA1-2023), and KAP1 (Bethyl Laboratories, A300-767A).

    Techniques: SDS Page, Quantitation Assay, Phospho-proteomics, Expressing

    (Step 1) DNA damage results in DSBs. (Step 2) SIRT2 deacetylates MRE11 at K393 in response to DNA damage. (Step 3) MRE11 K393 deacetylation by SIRT2 facilitates its recruitment and binding to DNA at DSBs. (Step 4) MRE11-RAD50-NBS1 assembles and promotes DNA end resection and ATM-dependent phosphorylation of downstream substrates, including CHK2 and KAP1.

    Journal: The Journal of Clinical Investigation

    Article Title: MRE11 deacetylation by SIRT2 promotes DNA binding to facilitate DNA end resection and ATM-dependent signaling

    doi: 10.1172/JCI186711

    Figure Lengend Snippet: (Step 1) DNA damage results in DSBs. (Step 2) SIRT2 deacetylates MRE11 at K393 in response to DNA damage. (Step 3) MRE11 K393 deacetylation by SIRT2 facilitates its recruitment and binding to DNA at DSBs. (Step 4) MRE11-RAD50-NBS1 assembles and promotes DNA end resection and ATM-dependent phosphorylation of downstream substrates, including CHK2 and KAP1.

    Article Snippet: The antibodies used in this study included SIRT2 (MilliporeSigma, 09-843; Santa Cruz Biotechnology, sc-20966), GAPDH (Santa Cruz, sc-47724), Flag (Santa Cruz Biotechnology, sc-51590), GFP (Abcam, Ab6556), pan acetyl-Lys (Santa Cruz Biotechnology, sc-8649), RPA70 (Cell Signaling Technology, 2267), P-CHK2 T68 (Cell Signaling Technology, 2661S), CHK2 (Santa Cruz Biotechnology, sc-7898), P-KAP1 (Invitrogen, Thermo Fisher Scientific, MA1-2023), and KAP1 (Bethyl Laboratories, A300-767A).

    Techniques: Binding Assay, Phospho-proteomics